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For The one histidine substitutions, the N61H mutant is comparable in catalytic action to the double mutant Whilst the Q59H mutant is diminished by below an purchase of magnitude relative to your wild kind enzyme. For that substitutions at Asn-sixty one, the replacement by having an alanine is significantly much less disruptive when compared to the improve to the histidine.
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m for your E131Q mutant is greater to 3.6 mM. A significant portion of this reduction in catalytic performance has arisen as the steel information on the purified proteins is relatively low.
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